Rooted phylogenetic tree derived from the deduced amino acid sequences of the concatenated genes.
The Israeli isolate of tomato brown rugose fruit virus (TBRFV-IL; KT721735); the Jordanian isolate of TBRFV (KT383474) and of several other viruses belonging to the genus Tobamovirus: Tomato mottle mosaic virus isolate MX5 (ToMMV-MX5; KF477193), Tomato mosaic virus isolate 1–2 (ToMV1-2; DQ873692), Tobacco mosaic virus (TMV; X68110), Tobacco mosaic virus isolate AH4 (TMV-AH4; KU321698), Tobacco mosaic virus isolate Oh (TMV-Oh; FR878069), Rehmannia mosaic virus isolate Jap (ReMV-Jap; AB628188), Rehmannia mosaic virus isolate Hen (ReMV-Hen; EF375551) and two outgroups: Pepper mild mottle virus (PMMoV; KX063611) and Tobacco mild green mosaic virus isolate Jap (TMGMV-Jap; AB078435). Each polyprotein-encoding sequence was aligned using the MAFT software for sequence alignments [26]. The tree was constructed based on maximum likelihood using the PhyML3.0 software with 1,000 bootstrap replicates [27].
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